微生物代谢国家重点实验室-科研队伍

杨广宇

姓名: 杨广宇

职称: 研究员

E-Mail: yanggy@sjtu.edu.cn

个人主页: http://enzyme.sjtu.edu.cn/yjry.asp

联系电话: 021-34205079

个人简历:

2003年毕业于吉林大学生命科学学院生物科学专业；同年进入吉林大学分子酶学工程教育部重点实验室开始硕博连读，2007年11月-2008年11月在加拿大不列颠哥伦比亚大学进行联合培养，2009年6月获生物化学与分子生物学博士学位。2009年7月至今在上海交通大学生命科学技术学院工作，2013年晋升为副研究员，2018年晋升为研究员。承担了生命学院《生物化学》、《蛋白质工程与应用》、《生物大分子结构与功能》的部分教学任务。

开展重要代谢酶的克隆与表征、酶结构-功能研究、分子设计与定向进化等研究工作，作为项目负责人承担了自然科学基金、973子课题、教育部博士点基金等科研项目，并作为主要成员参与了参与了多项国家973、863、自然科学基金项目的研究。在Nature Commun, Angew Chemie, J Am Chem Soc, Anal Chem, J Lipid Research等杂志发表论文34篇（其中第一及通讯作者21篇），他引430余次，申请及授权专利15项。担任了Scientific Reports, Applied and Environmental Microbiology, Journal of Biotechnology, Process Biochemistry等10余个SCI杂志的审稿人。2010、2013、2016年分别获上海交通大学“SMC晨星学者优秀青年教师奖励计划（C类）、（B类）、（A类）”支持。2015年获上海交通大学“青年岗位能手”称号。
1.酶功能及催化机制解析

2.酶分子设计与定向进化

3.体外合成生物学催化体系的构建
1. Fuqiang Ma^#, Meng Ting Chung^#, Yuan Yao, Robert Nidet, Lap Man Lee, Allen P. Liu, Yan Feng, Katsuo Kurabayashi*, Guang-Yu Yang*. Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform. Nature Communications, 2018, 9:1030

2. 马富强，杨广宇。基于液滴微流控技术的超高通量筛选体系及其在合成生物学中的应用。生物技术通报，2017，33(1):83-92.

3. Hoque MA, Zhang Y, Chen L, Yang G, Khatun MA, Chen H, Hao L, Feng Y. Stepwise Loop Insertion Strategy for Active Site Remodeling to Generate Novel Enzyme Functions. ACS Chemical Biology. 2017, 12(5):1188-1193.

4. Zhuang Y, Yang GY, Chen X, Liu Q, Zhang X, Deng Z, Feng Y. Biosynthesis of plant-derived ginsenoside Rh2 in yeast via repurposing a key promiscuous microbial enzyme. Metabolic Engineering. 2017, 42:25-32.

5. Han YB, Chen LQ, Li Z, Tan YM, Feng Y, Yang GY*. Structural insights into the broad substrate specificity of a novel Endo-glycoceramidase I belonging to a new subfamily of GH5 glycosidase. J Biol Chem. 2017, 292(12):4789-4800.

6. Fuqiang Ma, Yuan Xie, Manjie Luo, Shuhao Wang, You Hu, Yukun Liu, Yan Feng, Guang-Yu Yang*. Sequence Homolog-based Molecular Engineering for Shifting the Enzymatic pH Optimum. Synthetic and Systems Biotechnology, 2016, 1, 195-206

7. Zhang Y, An J, Yang G, Zhang X, Xie Y, Chen L, Feng Y. Structure features of GH10 xylanase from Caldicellulosiruptor bescii: implication for its thermophilic adaption and substrate binding preference. Acta Biochimica et Biophysica Sinica (ABBS). 2016, 48(10):948-957.

8. Fuqiang Ma, Michael Fischer, Yunbin Han, Stephen G. Withers, Yan Feng, Guang-Yu Yang*. Substrate engineering enabling fluorescence droplet entrapment for IVC-FACS-based ultrahigh-throughput screening. Analytical Chemisty, 2016, 88, 8587−8595

9. Xiao-Fei Zhang, Guang-Yu Yang, Yong Zhang, Yuan Xie, Stephen G. Withers, Yan Feng, A general and efficient strategy for generating the stable enzymes. Scientific Reports, 2016, 6, 33797

10. Xiaofei Zhang, Yong Zhang, Guangyu Yang, Yuan Xie, Lishi Xu, Jiao An, Li Cui, Yan Feng. Modulation of the thermostability and substrate specificity of Candida rugosa lipase1 by altering the acyl-binding residue Gly414 at the α-helix-connecting bend. Enzyme and Microbial Technology. 2016, 82, 34-41.

11. FT Huang, YB Han, Y Feng, GY Yang*. A facile method for controlling the reaction equilibrium of sphingolipid ceramide N-deacylase for lyso-glycosphingolipid production. Journal of lipid research. 2015, 56(9):1836-1842.

12. J An, Y Xie, Y Zhang, D Tian, S Wang, G Yang*, Y Feng*. Characterization of a thermostable, specific GH10 xylanase from Caldicellulosiruptor bescii with high catalytic activity. Journal of Molecular Catalysis B: Enzymatic. 2015, 117, 13-20.

13. Guang-Yu Yang, Caishun Li, Michael Fischer, Christopher W. Cairo, Yan Feng, Stephen G. Withers. A FRET Probe for Cell-Based Imaging of Ganglioside-Processing Enzyme Activity and High-Throughput Screening. Angewandte Chemie International Edition. 2015, 54(18):5389-5393

14. Yun-Bin Han, Lie Wu, Jamie R. Rich, Feng-Tao Huang, Stephen G. Withers, Yan Feng, Guang-Yu Yang*. Comprehensive Characterization of Sphingolipid Ceramide N-deacylase for the Synthesis and Fatty Acid Remodeling of Glycosphingolipids. Applied Microbiology and Biotechnology. 2015, 99(16):6715-6726.

15. Yu Zhang^#, Jiao An^#, Guangyu Yang^#, Aixi Bai, Baisong Zheng, Zhiyong Lou, Geng Wu, Wei Ye, Haifeng Chen, Yan Feng*, Giuseppe Manco* Active site loop conformation regulates promiscuous activity in a lactonase from Geobacillus kaustophilus HTA426 PloS One 2015, e0115130. (#equal contribution)

16. Xiaoli Zhou, Le Gao, Guangyu Yang, Donglai Liu, Aixi Bai, Binchun Li, Zixin Deng, Yan Feng* Design of hyperthermophilic lipase chimeras by key motif-directed recombination Chembiochem. 2015, 16, 455-462

17. Yuan Xie, Jiao An, Guangyu Yang, Geng Wu, Yan Feng*. Enhanced enzyme kinetic stability by increasing rigidity within the active site. J. Biol. Chem. 2014, 289, 7994-8006.

18. Fuqiang Ma, Yuan Xie, Chen Huang, Yan Feng, Guangyu Yang*. An improved single cell ultrahigh throughput screening method based on in vitro compartmentalization. PloS One. 2014, 9(2): e89785.

19. 罗漫杰，谢渊，钱志刚，冯雁，杨广宇*。超嗜热酯酶EST2在不同宿主中的异源高效表达研究。中国生物工程杂志，2014, 34(12), 36-44.

20. 施贤卫，张伟涛，张小飞，杨广宇，冯雁*。Actinosynnema mirum DSM 43827溶解性多糖单加氧酶的异源表达和酶学性质表征，中国生物工程杂志, 2014, 34(7):17-23.