由德林

姓名: 由德林

职称: 教授

E-Mail: dlyou@sjtu.edu.cn

个人主页:

联系电话: 62932943-2111

  • 个人简历:

    个人简介

    教授,致力于微生物分子酶学研究,研究领域涉及DNA磷硫酰化修饰和微生物天然产物生物合成。主要以表观遗传学领域的新技术和新方法,研究DNA磷硫酰化的生物化学途径和全基因组上的修饰序列特征,并深入探索这种新型表观遗传修饰在细胞防卫、基因表达调控等方面潜在的生理功能。以具有杂环结构微生物天然产物为研究对象,综合运用合成生物学、生物化学和分子遗传学等手段,开展微生物次级代谢产物的分离鉴定、生物合成基因簇的克隆、生物合成途径和调控机制及生物活性与功能的研究,并在此基础上开展活性天然产物的结构修饰、生物催化和组合生物合成改造。先后主持国家自然科学基金5项、863项目 3项、973项目子课题1项和上海市自然科学基金项目1项。

    教育经历

    1998.09-2001.07 博士,吉林大学

    1995.09-1998.07 硕士,吉林大学

    1987.09-1991.07 学士,长春工业大学

    工作经历

    2013.12- 现在 上海交通大学微生物代谢国家重点实验室,教授

    2003.10- 2013.12 上海交通大学微生物代谢国家重点实验室,副教授

    2010.08 -2011.08 美国麻省理工学院生物工程系,访问学者

    2001.10 -2003.10 北京大学生命科学学院,博士后

    1991.11-1998.10 吉林大学酶工程国家重点实验室

    荣誉奖励

    2012 明治生命科学奖(科学奖)

    2012 上海市浦江人才计划

    2008 SMC优秀青年教师奖(上海交通大学)

    2007 《环球科学》2007十大科学新闻

  • 1. DNA骨架的磷硫酰化修饰

    2. 微生物天然产物的生物合成

  • 1.       Cao B, Cheng Q, Gu C, Yao F, DeMott MS, Zheng X, Deng Z, Dedon PC, You D*. Pathological phenotypes and in vivo DNA cleavage by unrestrained activity of a phosphorothioate-based restriction system in SalmonellaMol Microbiol. 2014, DOI: 10.1111/mmi.12692.

    2.       Cao B, Chen C, DeMott MS, Cheng Q, Clark TA, Xiong X, Zheng X, Butty V, Levine SS, Yuan G, Boitano M, Luong K, Song Y, Zhou X, Deng Z, Turner SW, Korlach J, You D*, Wang L*, Chen S*, Dedon PC. Genomic mapping of phosphorothioates reveals partial modification of short consensus sequences.Nat Commun.  2014, 5: 3951.

    3.       Zhu T, Cheng X, Liu Y, Deng Z, You D*. Deciphering and engineering of the final step halogenase for improved chlortetracycline biosynthesis in industrial Streptomyces aureofaciens. Metab Eng. 2013, 19:69-78.

    4.       Zhang C, Kong L, Liu Q, Lei X, Zhu T, Yin J, Lin B, Deng Z, You D*. In vitro characterization of echinomycin biosynthesis: formation and hydroxylation of L-tryptophanyl-S-enzyme and oxidation of (2S,3S) β-hydroxytryptophan. PLoS One 2013, 8 (2): e56772.

    5.       Lei X, Kong L, Zhang C, Liu Q, Yao F, Zhang W, Deng Z, You D*In vivo investigation of the substrate recognition capability and activity affecting amino acid residues of glycosyltransferase FscMI in biosynthesis of candicidin. Mol BioSyst. 2013, 9 (3): 422-430.

    6.       Cao B, Yao F, Zheng X, Cui D, Shao Y, Zhu C, Deng Z, You D*. Genome mining of the biosynthetic gene cluster of the polyene macrolide antibiotic tetramycin and characterization of a P450 monooxygenase involved in the hydroxylation of the tetramycin B polyol segment. ChemBioChem 2012, 13(15):2234-42

    7.       Zhang W, Wang L, Kong L, Wang T, Chu Y, Deng Z, You D*. Unveiling the post-PKS redox tailoring steps in biosynthesis of the type II polyketide antitumor antibiotic xantholipin. Chem Biol. 2012, 19(3):422-32.

    8.       Liu Q, Yao F, Chooi Y, Kang Q, Xu W, Li Y, Shao Y, Shi Y, Deng Z, Tang Y*, You D*. Elucidation of piericidin A1 biosynthetic locus revealed a thioesterase-dependent mechanism of α-pyridone ring formation. Chem Biol. 2012, 19(2):243-53.

    9.       Wang T, Bai L, Zhu D, Lei X, Liu G, Deng Z, You D*. Enhancing macrolide production in Streptomyces by coexpressing three heterologous genes. Enzyme Microb.Tech. 2012, 50: 5-9.

    10.     Xu T, Yao F, Zhou X, Deng Z*, You D*. A novel host-specific restriction system associated with DNA backbone S-modification in Salmonella. Nucleic Acids Research. 2010, 38(20): 7133-41.

    11.     Yao F, Xu T, Zhou X, Deng Z*, You D*. Functional analysis of spfD gene involved in DNA phosphorothioation in Pseudomonas fluorescens Pf0-1. FEBS Lett. 2009, 583(4):729-33.

    12.     Chen W, Huang T, He X, Meng Q, You D, Bai L, Li J, Wu M, Li R, Xie Z, Zhou H, Zhou X, Tan H, Deng Z*. Characterization of the polyoxin biosynthetic gene cluster from Streptomyces cacaoi and engineered production of polyoxin H. J Biol Chem. 2009, 284(16): 10627-38.

    13.     Xu T, Liang J, Chen S, Wang L, He X, You D, Wang Z, Li A, Xu Z, Zhou X, Deng Z*. DNA phosphorothioation in Streptomyces lividans: mutational analysis of the dnd locus. BMC Microbiol. 2009, 9: 41.

    14.     Zhou Y, Meng Q, You D, Li J, Chen S, Ding D, Zhou X, Zhou H, Bai L, Deng Z*. Selective removal of aberrant extender units by a type II thioesterase for efficient FR-008/candicidin biosynthesis in Streptomyces sp. strain FR-008. Appl Environ Microbiol. 2008, 74(23):7235-42.

    15.     You D*, Xu T, Yao F, Zhou X, Deng Z*. Direct evidence that ThiI is an ATP pyrophosphatase for the adenylation of uridine in 4-thiouridine biosynthesis.ChemBioChem 2008, 9(12):1879-82.

    16.     Wang L, Chen S, Xu T, Taghizadeh K, Wishnok JS, Zhou X, You D, Deng Z*, Dedon PC*. Phosphorothioation of DNA in bacteria by dnd genes. Nat Chem Biol. 2007, 3(11): 709-10.

    17.     You D, Wang L, Yao F, Zhou X, Deng Z*. A novel DNA modification by sulfur: DndA is a NifS-like cysteine desulfurase capable of assembling DndC as an iron-sulfur cluster protein in Streptomyces lividans. Biochemistry. 2007, 46(20):6126-33.

    18.     Liu T, You D, Valenzano C, Sun Y, Li J, Yu Q, Zhou X, Cane DE, Deng Z*. Identification of NanE as the thioesterase for polyether chain release in nanchangmycin biosynthesis. Chem Biol. 2006, 13(9):945-55.

    19.     Wang L, Chen S, Xiao X, Huang X, You D, Zhou X, Deng Z*. arsRBOCT arsenic resistance system encoded by linear plasmid pHZ227 in Streptomyces sp. strain FR-008. Appl Environ Microbiol. 2006, 72(5):3738-42.

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