Ningyi Zhou

Ningyi Zhou



Phone: +86-21-34204135


Professor Ning-Yi Zhou received his Bachelor’s degree in Microbiology from Wuhan University and PhD in Microbiology from Imperial College London. Following postdoctoral training at Imperial College and University of Wales, Bangor, Prof. Zhou established his own laboratory in Wuhan Institute of Virology of Chinese Academy of Sciences. In 2013, he joined the at Shanghai Jiao Tong University as a distinguished professor and in 2015 he was appointed as the Head of Department of Microbial Sciences in the School of Life Science & Biotechnology. Over the last two decades, his research has been focusing on the genetics and biochemistry of microbial aromatic catabolism in various bacteria strains as well as the bioaugmentation of contaminated soil with microbial degraders, publishing more than 70 papers in this field. He was among the most cited Chinese researchers listed by Elsevier for three successive years from 2014 to 2016 (Microbiology & Immunology Section).

Currently, Prof Zhou is the head of the committee on Environmental Microbiology, Chinese Society for Microbiology. He is an editor for “Applied and Environmental Microbiology” and an associate editor for “Frontiers in Microbiology”.


1991-1995   Ph.D. in Microbiology, Department of Biochemistry, Imperial College of Science, Engineering and Medicine (now Imperial College London), UK.  Supervisor: Prof David Leak

1978-1982   BSc. in Microbiology, Department of Biology, Wuhan University

Work Experience

2013-   Distinguished Professor, School of Life Science & Biotechnology, Shanghai Jiao Tong University; Head of Department of Microbial Sciences (since 2015)

2000 -2016  Professor, Centre for Applied and Environmental MicrobiologyWuhan Institute of Virology, Chinese Academy of Sciences

1999-2000    Research Officer, School of Biological Sciences, University of Wales, Bangor (now Bangor University).  Supervisor: Prof Peter Williams

1996-1999   Postdoctoral research associate, Department of Biochemistry, Imperial College London.  Supervisor: Prof David Leak



1. Molecular biology and biochemistry of microbial catabolism of aromatics. 

2. Application of synthetic biology for pollution control and bioremediation

1. Gao YZ, Liu H, Chao HJ, and Zhou NY*. Constitutive expression of Nag-like dioxygenase gene through an internal promoter in the 2-chloronitrobenzene catabolic gene cluster of Pseudomonas stutzeri ZWLR2-1. Appl. Environ. Microbiol. 2016, 82:3461-3470.

2. Min J, Zhang JJ, Zhou NY*.  A Two-Component para-nitrophenol monooxygenase initiates a novel 2-chloro-4-nitrophenol catabolism pathway in Rhodococcus imtechensis RKJ300. Appl. Environ. Microbiol. 2016, 82: 714-723.

3. Chao HJ, Chen YF, Fang T, Xu Y, Huang WE, Zhou NY*. HipH catalyzes the hydroxylation of 4-hydroxyisophthalate to protocatechuate in 2, 4-xylenol catabolism by pseudomonas putida NCIMB 9866. Appl. Environ. Microbiol. 2016, 82: 724-731.

4. Liu S, Su T, Zhang C, Zhang, WM, Zhu D, Su J, Wei T, Wang K, Huang Y, Guo L, Xu S, Zhou NY*, Gu L*. Crystal structure of PnpCD, a two-subunit hydroquinone 1, 2-dioxygenase, reveals a novel structural class of Fe2+-dependent dioxygenases. J Biol Chem, 2015, 290: 24547-24560.

5. Liu K Xu Y, Zhou NY*. Identification of a specific maleate hydratase in the direct hydrolysis route of the gentisate pathway. Appl. Environ. Microbiol. 2015, 81: 5753-5760

6. Zhang WM, Zhang JJ, Jiang X, Chao HJ, Zhou NY*. Transcriptional activation of multiple operons involved in para-nitrophenol degradation by Pseudomonas sp. strain WBC-3. Appl. Environ. Microbiol. 2015, 81: 220-230

7. Min J, Zhang JJ, and Zhou NY*. The gene cluster for para-nitrophenol catabolism is responsible for 2-chloro-4-nitrophenol degradation in Burkholderia sp. strain SJ98. Appl. Environ. Microbiol. 2014, 80: 6212-6222

8. Chao HJ, Zhou NY*. Involvement of the global regulator GlxR in 3-hydroxybenzoate and gentisate utilization by Corynebacterium glutamicum. Appl. Environ. Microbiol. 2014, 80:4215-4225

9. Xu Y, Chen B, Chao HJ, Zhou NY*. 2013. mhpT encodes an active transporter involved in 3-(3-hydroxyphenyl)propionate catabolism by Escherichia coli K-12. Appl. Environ. Microbiol. 2013, 79: 6362-6368

10. Sun DL, Jiang X, Wu QL, Zhou NY*. Intragenomic heterogeneity of 16S rRNA genes causes overestimation of prokaryotic diversity. Appl. Environ. Microbiol. 2013, 79:5962-5969

11. Chao HJ, Zhou NY*. GenR, an IclR-type regulator, activates and represses the transcription of gen genes involved in 3-hydroxybenzoate and gentisate catabolism in Corynebacterium glutamicum. J. Bacteriol. 2013, 195: 1598-1609

12. Liu K, Liu TT, Zhou NY*. HbzF catalyzes direct hydrolysis of maleylpyruvate in the gentisate pathway of Pseudomonas alcaligenes NCIMB 9867. Appl. Environ. Microbiol. 2013, 79: 1044-1047

13. Chi XQ, Zhang JJ, Zhao S, Zhou NY*. Bioaugmentation with a consortium of bacterial nitrophenol-degraders for remediation of soil contaminated with three nitrophenol isomers. Environ. Pollut.2013, 172:33-41

14. Liu TT , Zhou NY*. Novel L-cysteine dependent maleylpyruvate isomerase in the gentisate pathway of Paenibacillus sp. strain NyZ101. J. Bacteriol. 2012, 194:3987-3994

15. Xiao Y, Liu TT, Dai H, Zhang JJ, Liu H, Tang H, Leak DJ, Zhou NY*. OnpA, an unusual flavin dependent monooxygenase containing a cytochrome b5 domain. J. Bacteriol. 2012, 194: 1342-1349

16. Xu Y, Gao X, Wang SH, Liu H, Williams PA, Zhou NY*. MhbT is a specific transporter for 3-hydroxybenzoate uptake by Gram-negative bacteria. , Appl. Environ. Microbiol. 2012, 78:6113-6120

17. Liu H, Wang SJ, Zhang JJ, Dai H, Tang H, and Zhou NY*. Patchwork assembly of nag-like nitroarene dioxygenase genes and 3-chlorocatechol degradation cluster for the pathway evolution of 2-chloronitrobenzene catabolism in Pseudomonas stutzeri ZWLR2-1. Appl Environ Microbiol, 2011, 77:4547-4552.

18. Zhang JJ, Liu H, Xiao Y, Zhang XE, Zhou NY*. Identification and characterization of catabolic para-nitrophenol 4-monooxygenase and para-benzoquinone reductase from Pseudomonas sp. strain WBC-3. J Bacteriol, 2009, 191:2703-2710.

19. Zhao S, Ramette A, Niu GL, Liu H, Zhou NY*. Effects of nitrobenzene contamination and of bioaugmentation on nitrification and ammonia-oxidizing bacteria in soil. FEMS Microbiol Ecol, 2009, 70:315-323.

20. Xiao Y, Zhang JJ, Liu H, Zhou NY*. Molecular characterization of a novel ortho-nitrophenol catabolic gene cluster in Alcaligenes sp. strain NyZ215. J Bacteriol, 2007, 189:6587-6593.

21. Yan DZ, Liu H, Zhou NY*.Conversion of Sphingobium chlorophenolicum ATCC 39723 to a hexachlorobenzene degrader by metabolic engineering. Appl Environ Microbiol, 2006, 72(3):2283-2286.

22. Zhou NY, Al-Dulayymi J, Baird MS, and Williams PA*. Salicylate 5-hydroxylase from Ralstonia sp. strain U2: a monooxygenase with close relationships to and shared electron transport proteins with naphthalene dioxygenase. J Bacteriol. 2002, 184:1547-1555. 

23. Zhou NY. Fuenmayor SL, and Williams PA*. nag genes of Ralstonia (formerly Pseudomonas) sp. strain U2 encoding enzymes for gentisate catabolism. J Bacteriol. 2001, 183:700-708.

24. Zhou NY, Jenkins A, Chan Kwo Chion C K, Leak DJ*. The alkene monooxygenase from Xanthobacter strain Py2 is closely related to aromatic monooxygenases and catalyzes aromatic monohydroxylation of benzene, toluene, and phenol. Appl Environ Microbiol 1999, 65:1589-1595.



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